Extraction of Biologically Active Substances from the Bark and Leaves of Trees of the Salicaceae Family
DOI:
https://doi.org/10.37482/0536-1036-2025-4-173-184Keywords:
Salicaceae, extraction, processing, biologically active substances, salicin, quercetin, leaves, barkAbstract
The process of extraction of biologically active substances from non-woody parts of trees (bark and leaves) of willow and aspen is described in the article. The instrumentation for extracting salicin and quercetin from the bark and leaves of trees of the Salicaceae family is presented. A method for experimental research and obtaining of biologically active substances is presented. It has been found that an increase in the extractant consumption from 20 to 100 l/min at the same temperature (75 °С) and 95 % impregnation leads to a 2-fold acceleration of the process. Local temperature changes across the layers of the material have been described. A slight lag in heating of subsequent layers of the material by 1–2 min has been determined, which is due to the thermal inertia of the material and the peculiarities of heat transfer within the layer. It takes 7 min to achieve full heating. A kinetic curve of the concentration of extracted biologically active substances in the extract has been constructed. The resulting dependencies and comparative data allow us to recommend rational modes of extraction of biologically active substances from the bark and leaves of willow and aspen trees. According to the results of the research, the optimal temperature for quercetin extraction has been recommended to be 50 °С. It has also been found that increasing the temperature to 75 °С leads to a noticeable increase in the yield of salicin. Moreover, it has been revealed that the largest amount of quercetin is found in aspen (1.4 %), with 11.4 % more quercetin in the leaves than in the bark; the main amount of salicin is found in the bark of both species, but in willow bark there is an excess of 35 %; the rational solvent concentration is 60 %; the duration of extraction is 40–45 min.
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А. с. СССР 986380. Способ переработки осиновой коры / Л.А. Гришкова, Е.А. Демченко, В.Е. Ковален, В.Я. Ланд, И.И. Маркичев, В.Б. Некрасова. Grishkova L.A., Demchenko E.A., Kovalen V.E., Land V.Ya., Markichev I.I., Nekrasova V.B. Method of Processing Aspen Bark: Author’s Certificate USSR 986380. (In Russ.).
Блажей А., Шутый Л. Фенольные соединения растительного происхождения. М.: Мир, 1977. 240 с. Blazhej A., Shutyj L. Phenolic Compounds of Plant Origin. Moscow, Mir Publ., 1977. 240 p. (In Russ.).
Дементьева Т.М. Изучение коры и побегов ивы вавилонской (Salix babylonica L.) и гибрида ивы вавилонской и ивы белой (Salix babylonica L. × Salix alba L.), произрастающих на Северном Кавказе: дис. ... канд. фарм. Наук. Пятигорск, 2016. 154 с. Dement’eva T.M. Study of the Bark and Shoots of the Babylonian Willow (Salix babylonica L.) and the Hybrid of the Babylonian Willow and the White Willow (Salix babylonica L. × Salix alba L.), Growing in the North Caucasus: Cand. Diss. Pharm. Sci. Diss., 2016. 154 p. (In Russ.).
Демченкова Е.Ю. Стандартизация лекарственного растительногосырья и фитопрепаратов на содержание антиоксидантов амперометрическим методом: автореф. дис. ... канд. фарм. наук. Москва, 2012. 25 с. Demchenkova E.Yu. Standardization of Medicinal Plant Raw Materials and Phytopreparations for Antioxidant Content Using the Amperometric Method: Cand. Pharm. Sci. Diss. Abs. Moscow, 2012. 25 p. (In Russ.).
Жматова Г.В., Нефедов А.Н., Гордеев А.С., Килимник А.В. Методы интенсификации технологических процессов экстрагирования биологически активных веществ из растительного сырья // Вестн. ТГТУ. 2005. Т. 11, No 3. С. 701–707. Zhmatova G.V., Nefedov A.N., Gordeev A.S., Kilimnik A.B. Intensification Methods of Extracting Biologically Active Substances from Vegetative Raw Materials. Vestnik TGTU = Transactions TSTU, 2005, vol. 11, no. 3, pp. 701–707. (In Russ.).
Литвиненко В.И. Химия природных флавоноидов и создание препаратов при комплексной переработке растительного сырья: автореф. дис. ... д-ра хим. наук. Харьков: ХНИИХФИ, 1990. 29 с. Litvinenko V.I. Chemistry of Natural Flavonoids and Creation of Preparations During Complex Processing of Plant Raw Materials: Doc. Chem. Sci. Diss. Abs. Kharkiv, Kharkiv Research Chemical-Pharmaceutical Institute, 1990. 29 p. (In Russ.).
Ломако Е.В., Кузьмичева Н.А. Применение поверхностно-активных веществ в анализе лекарственного растительного сырья, содержащего флавоноиды // Вестн. фармации. 2014. No 3 (65). С. 42–49. Lomako E.V., Kuzmichova N.A. Application of Surfactants in the Analysis of Medicinal Vegetative Raw Material Containing Flavonoids. Vestnik farmatsii, 2014, no. 3 (65), pp. 42–49. (In Russ.).
Масленникова К.А., Конюхова О.М., Канарский А.В. Фенолгликозиды растений семейства Salicaceae // Вестн. Казанск. технол. ун-та. 2014. T. 17, No 14. С. 383–386. Maslennikova K.A., Konyukhova O.M., Kanarskii A.V. Phenolglycosides of Plants of the Salicaceae Family. Vestnik Kazanskogo tekhnologicheskogo universtiteta = Herald of Technological University, 2014, vol. 17, no. 14, pp. 383–386. (In Russ.).
Матвеенко Е.В., Величко Н.А., Ушанов С.А., Айошина Е.Н. Математическая модель процесса экстракции древесной зелени Juniperus sibirica burgsd водным раствором этилового спирта // Проблемы современной аграрной науки: материалы Междунар. заоч. науч. конф. Красноярск, 2015. С. 169–174. Matveenko E.V., Velichko N.A., Ushanov S.V., Ayoshina E.N. Mathematical Model of the Extraction Process of Juniperus sibirica burgsd Wood Greenery with an Aqueous Solution of Ethyl Alcohol. Problems of Modern Agricultural Science: Proceedings of the International Correspondence Scientific Conference. Krasnoyarsk, 2015, pp. 169–174. (In Russ.).
Пояркова Н.М., Сапарклычева С.Е. Физиологическая роль фенольных соединений // Аграрн. образование и наука. 2019. No 4. Режим доступа: https://aes.urgau.ru/ru/4-2019/32-4-2019 (дата обращения: 20.06.25). Poyarkova N.M., Saparklycheva S.E. The Physiological Role of Phenolic Compounds. Agrarnoe obrazovanie i nauka = Agrarian Education and Science, 2019, no. 4. (In Russ.).
Рассыпнова С.С., Турецкова В.Ф., Зверев Я.Ф. Изучение противовоспалительного действия экстракта из коры Populus tremula (Salicaceae) и входящих в его состав фенольных соединений // Растит. ресурсы. 2010. Т. 46, No 3. С. 103–108. Rassypnova S.S., Turetskova V.F., Zverev Ya.F. Study of the Anti-Inflammatory Effect of Populus tremula (Salicaceae) Bark Extract and its Constituent Phenolic Compounds. Rastitelnye Resursy, 2010, vol. 46, no. 3, pp. 103–108. (In Russ.).
Сафин Р.Г., Просвирников Д.Б., Арсланова Г.Р., Валеев К.В., Зиатдинова Д.Ф., Гурьянов Д.А. Математическое описание процесса экстракции фенольных соединений // Деревообрабатывающая промышленность. 2022. No 1. С. 62–70. Safin R.G., Prosvirnikov D.B., Arslanova G.R., Valeev K.V., Ziatdinova D.F., Guryanov D.A. Mathematical Description of the Extraction Process of Phenolic Compounds. Derevoobrabativaushaya promishlennost’ = Woodworking Industry, 2022, no. 1, pp. 62–70. (In Russ.).
Ушанова В.М., Лебедева О.И., Девятловская А.Н. Основы научных исследований. Ч. 2. Контроль качества и экстрагирование растительного сырья. Красноярск: СибГТУ, 2004. 168 с. Ushanova V.M., Lebedeva O.I., Devyatlovskaya A.N. Fundamentals of Scientific Research. Part 2. Quality Control and Extraction of Plant Raw Materials. Krasnoyarsk, Siberian State Technological University Publ., 2004. 168 p. (In Russ.).
Швец П., Халабала М. Кислота ацетилсалициловая – лекарство, проверенное поколениями (к столетию ацетилсалициловой кислоты) // Словакофарма ревю. Киев, 2002. С. 66–68. Shvets P., Khalabala M. Acetylsalicylic Acid – a Medicine Tested by Generations (on the Centenary of Acetylsalicylic Acid). Slovakofarma revu. Kyiv, 2002, pp. 66–68. (In Russ.).
Шегельман И.Р., Кузнецов А.В. Эффективное использование лесных ресурсов. Петрозаводск: ПетрГУ, 2008. 88 с. Shegel’man I.R., Kuznetsov A.V. Efficient Use of Forest Resources. Petrozavodsk, Petrozavodsk State University, 2008. 88 p. (In Russ.).
Jokić S., Jerković I., Rajić M., Aladić K., Bilić M., Vidović S. SC-CO2 Extraction of Vitex agnus-castus L. Fruits: The Influence of Pressure, Temperature and Water Presoaking on the Yield and GC–MS Profiles of the Extracts in Comparison to the Essential Oil Composition. The Journal of Supercritical Fluids, 2017, vol. 123, pp. 50–57. https://doi.org/10.1016/j.supflu.2016.12.007
Safina A.V., Valeev K.V., Ziatdinova D.F. Method for Determining the Mass Conductivity Coefficient of Wood when Extracting Biologically Active Substances from it. Journal of Physics: Conference Series, 2022, vol. 2373, art. no. 042004. https://doi.org/10.1088/1742-6596/2373/4/042004
Safin R.G., Prosvirnikov D.B., Arslanova G.R. Parametric Multidimensional Modeling of Extraction Processes in the Wood Chemical, Food and Pharmaceutical Industries. Proceedings of the 7th International Conference on Industrial Engineering (ICIE 2021). Springer, Cham, 2021, pp. 286–297. https://doi.org/10.1007/978-3-030-85230-6_34
Soares J.F., Zabot G.L., Tres M.V., Lunelli F.C., Rodrigues V.M., Friedrichc M.T., Pazinatto C.A., Bilibio D., Mazutti M.A., Carniel N., Priamo W.L. Supercritical CO2 Extraction of Black Poplar (Populus nigra L.) Extract: Experimental Data and Fitting of Kinetic Parameters. The Journal of Supercritical Fluids, 2016, vol. 117, pp. 270–278. https://doi.org/10.1016/j.supflu.2016.07.005
