Applying Sulfite Liquors as a Nutrient Medium for Cultivation of Lactic Acid Producer Rhizopus oryzae F-1030
DOI:
https://doi.org/10.37482/0536-1036-2021-5-163-173Keywords:
sulphite liquor, Rhizopus oryzae, lactic acid, cellulase, xylanase, semicontinuous culture method, batch culture methodAbstract
The filamentous fungi Rhizopus oryzae are well known for their ability to hydrolyze plant polysaccharides. Representatives of this species are widely used for the production of various products, such as organic acids (lactic acid, fumaric acid), ethanol, and hydrolytic enzymes (glucoamylases, polygalacturonases). Various carbon sources such as pentose and hexose sugars are used for the synthesis of R. oryzae exoproducts. Expensive plant sugarcontaining raw materials are used in the lactic acid production by the microbiological method, which significantly increases the final cost of the product. The article demonstrates the possibility of using cheaper by-products of pulp and paper production such as sulphite liquors as an alternative source of carbon for microbiological synthesis of lactic acid. Sulphite liquors contain a large amount of carbohydrates – products of incomplete hydrolysis of cellulose such as hemicelluloses, mono- and oligomeric sugars. The composition of sulfite liquors allows us to consider them as a potential substrate for the synthesis of lactic acid by using different microorganisms. The paper considers the dependence of the lactic acid yield on the composition of the nutrient medium based on sulfite liquor and the cultivation method of the fungus R. oryzae F-1030. As additional sources of nitrogen and phosphorus, (NH4)2SO4 and KH2PO4 were introduced to the nutrient medium. The fungus R. oryzae F-1030 was cultivated by two methods: the semicontinuous culture method, when the nutrient medium was completely replaced after depletion of the reducing sugars in it; the batch culture method, when the lack of reducing sugars in the medium was compensated by the addition of concentrated sulfite liquor. Since the nutrient medium based on sulfite liquor contains the products of incomplete cellulose hydrolysis and xylans, the xylanase and cellulase activities of the fungus R. oryzae F-1030 were measured in order to determine its absorption degree of carbohydrates, contained in the nutrient medium. It was found that it is more expedient to use the semicontinuous method for the fungus R. oryzae F-1030 cultivation on a nutrient medium based on sulfite liquor in order to obtain more synthesized lactic acid. The addition of mineral sources of nitrogen and phosphorus has practically no effect on the final yield of lactic acid.
For citation: Mingazova L.A., Kryakunova E.V., Kanarskaya Z.A., Kanarsky A.V. Applying Sulfite Liquors as a Nutrient Medium for Cultivation of Lactic Acid Producer Rhizopus oryzae F-1030. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 5, pp. 163–173. DOI: 10.37482/0536-1036-2021-5-163-173
Acknowledgements: The work was carried out using the equipment of the Center for Collective Use “Ecology, Biotechnology and Processes for Producing Environmentally Friendly Energy Carriers” of the Volga State University of Technology.
Downloads
References
Борщевская Л.Н., Гордеева Т.Л., Калинина А.Н., Синеокий С.П. Спектрофотометрическое определение молочной кислоты // Журнал аналит. химии. 2016. Т. 71, № 8. С. 787–790. Borshchevskaya L.N., Gordeeva T.L., Kalinina A.N., Sineokii S.P. Spectrophotometric Determination of Lactic Acid. Zhurnal analiticheskoy khimii [Journal of Analytical Chemistry], 2016, vol. 71, no. 8, pp. 787–790. DOI: https://doi.org/10.7868/S004445021608003X
Евелева В.В., Новицкая И.Б., Черпалова Т.М., Никулина И.Д. Технологические инновации в производстве пищевой молочной кислоты // Пищевая пром-сть. 2014. № 4. С. 26–28. Eveleva V.V., Novitskaya I.B., Cherpalova T.M., Nikulina I.D. Technological Innovations in Production of Food Lactic Acid. Pishchevaya promyshlennost’ [Food Industry], 2014, no. 4, pp. 26–28.
Коренман И.М. Фотометрический анализ. Методы определения органических соединений. М.: Химия, 1970. 343 с. Korenman I.M. Photometric Analysis. Methods for Determination of Organic Compounds. Moscow, Khimiya Publ., 1970. 343 p.
Мингазова Л.А., Канарский А.В., Крякунова Е.В., Канарская З.А. Синтез молочной кислоты грибом Rhizopus oryzae F-1030 на питательных средах из сульфитных щелоков // Изв. вузов. Лесн. журн. 2020. № 2. С. 146–158. Mingazova L.A., Kanarsky A.V., Kryakunova E.V., Kanarskaya Z.A. Lactic Acid Synthesis by Fungus Rhizopus oryzae F-1030 on Growth Media Based on Sulphite Liquors. Lesnoy Zhurnal [Russian Forestry Journal], 2020, no. 2, pp. 146–158. DOI: https://doi.org/10.37482/0536-1036-2020-2-146-158
Морозова Ю.А., Скворцов Е.В., Алимова Ф.К., Канарский А.В. Биосинтез ксиланаз и целлюлаз грибами рода Trichoderma на послеспиртовой барде // Вестн. Казан. технол. ун-та. 2012. Т. 15, № 19. С. 120–122. Morozova Yu.A., Skvortsov E.V., Alimova F.K., Kanarsky A.V. The Biosynthesis of Xylanase and Cellulase by Fungi of Trichoderma Genus on DDGS. Vestnik Kazanskogo tekhnologicheskogo universiteta [Bulletin of the Technological University], 2012, vol. 15, no. 19, pp. 120–122.
Намханов В.В., Будаев Б-Ж.А., Товаршинов А.И., Борбоев Л.В. Влияние материалов зубных протезов на органы полости рта // Вестн. БГУ. 2009. № 12. C. 143–149. Namhanov V.V., Budaev B-Zh.A., Tovarshinov A.I., Borboev L.V. Influence of Materials of Dental Artificial Limbs on Bodies of Oral Cavity. Vestnik buryatskogo gosuniversiteta [BSU bulletin], 2009, no. 12, pp. 143–149.
Никанова Л.А. Влияние органических кислот на продуктивность, резистентность, микробиоценоз кишечника и биохимические показатели сыворотки крови свиней // Достижения науки и техники АПК. 2018. Т. 32, № 7. С. 65–67. Nikanova L.A. Influence of Organic Acids on Productivity, Resistance, Intestinal Microbiocenosis and Biochemical Parameters of Blood Serum of Pigs. Dostizheniya nauki i tekhniki APK [Achievements of Science and Technology of AIC], 2018, vol. 32, no. 7, pp. 65–67. DOI: 10.24411/0235-2451-2018-10715
Новожилов Е.В. Оценка биоресурса сульфитных щелоков как сырья для производства кормовых дрожжей // Изв. вузов. Лесн. журн. 1999. № 2-3. С. 179–188. Novozhilov E.V. Assessment of Sulfite Liquors Bioresource as a Raw Material for Nutrient Yeast Production. Lesnoy Zhurnal [Russian Forestry Journal], 1999, no. 2-3, pp. 179–188. URL: http://lesnoizhurnal.ru/upload/iblock/eea/179_188.pdf
Няникова Г.Г., Комиссарчик С.М., Хрусталёва М.В. Исследование условий культивирования Rhizopus oryzae для получения молочной кислоты и биосорбента // Изв. СПбГТИ (ТУ). 2012. Т. 17, № 43. С. 56–60. Nyanikova G.G., Komissarchik S.M., Khrustaleva M.V. A Study of the Rhizopus oryzae Cultivation Conditions for Lactic Acid and Biosorbent Preparation. Izvestiya SPbGTI (TU) [Bulletin of St PbSIT (TU)], 2012, vol. 17, no. 43, pp. 56–60. DOI: https://doi.org/10.1002/chin.201240145
Самуйленко А.Я., Гринь С.А., Еремец В.И., Шинкарев С.М., Неминущая Л.А., Скотникова Т.А., Лермонтов С.А., Зимагулова Л.А., Галиева А.Р. Тенденции развития производства молочной кислоты // Вестн. технол. ун-та. 2017. Т. 20, № 1. С. 162–166. Samujlenko A.Ya., Grin S.A., Eremets V.I., Shinkarev S.M., Neminuschiy L.A., Skotnikova T.A., Lermontov S.A., Zimagulova L.A., Galieva A.R. Lactic Acid Production Trends. Vestnik tekhnologicheskogo universiteta [Bulletin of the Technological University], 2017, vol. 20, no. 1, pp. 162–166.
Филиппова В.Н. Фруктовые кислоты. Их роль в косметике // Сервис в России и за рубежом. 2007. Т. 2, № 2. C. 163–165. Filippova V.N. Fruit Acids and Their Role in Cosmetics. Servis v Rossii i za rubezhom [Services in Russia and abroad], 2007, vol. 2, no. 2, pp. 163–165.
Шарков В.И., Сапотницкий С.А., Дмитриева О.А., Туманов И.Ф. Технология гидролизных производств. М.: Лесн. пром-сть, 1973. 407 с. Sharkov V.I., Sapotnitskiy S.A., Dmitriyeva O.A., Tumanov I.F. Technology of Hydrolysis Production. Moscow, Lesnaya promyshlennost ʼ Publ., 1973. 407 p.
Abd Alsaheb R.A., Aladdin A., Othman N.Z., Malek R.A., Leng O.M., Aziz R., El Enshasy H.A. Lactic Acid Applications in Pharmaceutical and Cosmeceutical Industries. Journal of Chemical and Pharmaceutical Research, 2015, vol. 7, iss. 10, pp. 729–735.
Abdel-Rahman M.A., Tashiro Y., Sonomoto K. Recent Advances in Lactic Acid Production by Microbial Fermentation Processes. Biotechnology Advances, 2013, vol. 31, iss. 6, pp. 877–902. DOI: https://doi.org/10.1016/j.biotechadv.2013.04.002
Abdel-Rahman M.A., Xiao Y., Tashiro Y., Wang Y., Zendo T., Sakai K., Sonomoto K. Fed-Batch Fermentation for Enhanced Lactic Acid Production from Glucose/Xylose Mixture without Carbon Catabolite Repression. Journal of Bioscience and Bioengineering, 2015, vol. 119, iss. 2, pp. 153–158. DOI: https://doi.org/10.1016/j.jbiosc.2014.07.007
Adney B., Baker J. Measurement of Cellulase Activities. Technical Report NREL/TP-510-42628. Golden, CO, National Renewable Energy Laboratory, 2008. 8 p.
Ahring B.K., Traverso J.J., Murali N., Srinivas K. Continuous Fermentation of Clarified Corn Stover Hydrolysate for the Production of Lactic Acid at High Yield and Productivity. Biochemical Engineering Journal, 2016, vol. 109, pp. 162–169. DOI: https://doi.org/10.1016/j.bej.2016.01.012
Bailey M.J., Biely J., Poutanen K. Interlaboratory Testing of Methods for Assay of Xylanase Activity. Journal of Biotechnology, 1992, vol. 23, iss. 3, pp. 257–270. DOI: https://doi.org/10.1016/0168-1656(92)90074-J
Choudhary J., Singh S., Nain L. Thermotolerant Fermenting Yeast for Simultaneous Saccharification and Fermentation of Lignocellulosic Biomass. Electronic Journal of Biotechnology, 2016, vol. 21, pp. 82–92. DOI: https://doi.org/10.1016/j.ejbt.2016.02.007
Gao C., Ma C., Xu P. Biotechnological Routes Based on Lactic Acid Production from Biomass. Biotechnology Advances, 2011, vol. 29, is. 6, pp. 930–939. DOI: https://doi.org/10.1016/j.biotechadv.2011.07.022
Hofvendahl K., Hahn-Hägerdal B. Factors Affecting the Fermentative Lactic Acid Production from Renewable Resources. Enzyme and Microbial Technology, 2000, vol. 26, iss. 2-4, pp. 87–107. DOI: https://doi.org/10.1016/S0141-0229(99)00155-6
Kleerebezem R., van Loosdrecht M.C.M. Mixed Culture Biotechnology for Bioenergy Production. Current Opinion in Biotechnology, 2007, vol. 18, iss. 3, pp. 207–212. DOI: https://doi.org/10.1016/j.copbio.2007.05.001
Komesu A., Rocha de Oliveira J.A., da Silva Martins L.H., Wolf Maciel M.R., Maciel Filho R. Lactic Acid Production to Purification: A Review. BioResources, 2017, vol. 12, no. 2, pp. 4364–4383. DOI: https://doi.org/10.15376/biores.12.2.Komesu
Nakano S., Ugwu C.U., Tokiwa Y. Efficient Production of D-(–)-Lactic Acid from Broken Rice by Lactobacillus delbrueckii Using Ca(OH)2 as a Neutralizing Agent. Bioresource Technology, 2012, vol. 104, pp. 791–794. DOI: https://doi.org/10.1016/j.biortech.2011.10.017
Nancib A., Nancib N., Boudrant J. Production of Lactic Acid from Date Juice Extract with Free Cells of Single and Mixed Cultures of Lactobacillus casei and Lactococcus lactis. World Journal of Microbiology and Biotechnology, 2009, vol. 25, pp. 1423–1429. DOI: https://doi.org/10.1007/s11274-009-0029-z
Ofongo R.T.S., Ohimain E.I., Iyayi E.A. Cellulase and Hemicellulase Activity under Submerged Fermentation of Rice Mill Feed by Fungi. International Journal of Environment, Agriculture and Biotechnology, 2019, vol. 4, iss. 1, pp. 233–239. DOI: https://doi.org/10.22161/ijeab/4.1.35
Taherzadeh M.J., Fox M., Hjorth H., Edebo L. Production of Mycelium Biomass and Ethanol from Paper Pulp Sulfite Liquor by Rhizopus oryzae. Bioresource Technology, 2003, vol. 88, iss. 3, pp. 167–177. DOI: https://doi.org/10.1016/S0960-8524(03)00010-5
Wee Y.-J., Kim J.-N., Ryu H.-W. Biotechnological Production of Lactic Acid and Its Recent Applications. Food Technology and Biotechnology, 2006, vol. 44, no. 2, pp. 163–172.
Yadav A.K., Chaudhari A.B., Kothari R.M. Bioconversion of Renewable Resources into Lactic Acid: An Industrial View. Critical Reviews in Biotechnology, 2011, vol. 31, no. 1, pp. 1–19. DOI: https://doi.org/10.3109/07388550903420970
